Experiment design.

Pregnant female (F0) mice were exposed five hours a day, five days a week throughout gestation days (GD) 0–21 (n = 60 offspring from PAH exposure vs. negative control aerosol, n = 46 dams per group). Beginning PND21, n = 38 in PAH and n = 53 offspring (F1) from 11 litters were weighed every other day. Beginning PND28, total n = 134 mice from 28 litters were weighed every other day. On PND 60, n = 13 female mice from 12 litters in F1 were mated to generate F2 mice. From the remaining mice, n = 21 mice following PAH, and n = 18 mice following negative control were assessed for body fat composition, and fat mass for WAT and BAT at PND60 ±2.46 of age, and gene expression and DNA methylation were measured. Similar outcomes at the same time point (PND60) were measured in F2 mice.