Effect of ail alleles on Ail protein abundance.

1Ratio of protein abundance of evolved population relative to ancestor. Error reflects standard deviation of the mean using triplicate technical replicates.2Insertion sequence mediated mutations are referred to by their nucleotide position (nt, nucleotide).3Ancestor only, protein was observed in ancestor but not in evolved population.4Indel mutations resulting in frame shift and premature stop codon are indicated by the relevant amino acid mutation* (bp, base pair).5Protein was only identified in one of three technical replicates; therefore statistical analysis of variance was impossible.Effect of ail alleles on Ail protein abundance.