File S1 - True-Breeding Targeted Gene Knock-Out in Barley Using Designer TALE-Nuclease in Haploid Cells

Figures S1–S5 and Tables S1–S2. Figure S1: Embryogenic pollen cultures developed from donor gfp transgenic barley. Figure S2: DNA gel blot and gfp expression analysis of the donor line PV89. Pollen culture of independent hemizygous transgenic plant is shown as a control. Figure S3: PCR amplification of the FokI domain from a template of cDNA prepared from each of the primary gfp mutants, the gfp donor and non-transformed cv. ‘Igri’. ACTIN served as a positive control. Figure S4: Best matches found in the barley genome as compared to the target sequence. Figure S5: Immature pollen produced by primary transgenic gfp knock-out plant 24/2-1 and gfp donor. Table S1: Primer sequences used for the identification of T-DNA elements. Table S2: Transgenic plants characterized for ploidy level and the presence and expression of TALEN T-DNA. Sequences of the used binary vectors. (DOC)