"Revealing intrinsic limitations of immobilized-laccase systems, questioning their relevance for mediator-free organic pollutants oxidation"

Calibration curve of A420 for fully oxidized ABTS solutionsHPLC calibration curves for APAP and BPA quantificationEnzymatic activity measured in solution before and after PCm-lac synthesis; enzyme loading calculated from the difference between initial and final activityTime-dependent activity of soluble and immobilized laccase; stability expressed as percentage of initial activityHPLC-monitored degradation of APAP and BPA by free laccase and PCm-lacResidual PCm-lac activity following APAP and BPA degradation experimentsMechanistic investigation of soluble laccase inactivation during BPA oxidation through simultaneous monitoring of BPA concentration and enzymatic activity