Lactobacillus johnsonii suppresses colorectal tumorigenesis by enriching the intestinal metabolite to induce tumor cell apoptosis

Lactobacillus johnsonii (LJ) has demonstrated therapeutic potential in various human diseases, but its direct impact on colorectal cancer (CRC) remains insufficiently explored. This study evaluated the prophylactic and therapeutic effects of LJ on colorectal tumors using comprehensive experimental models. Azoxymethane/dextran sulfate sodium (AOM/DSS)-induced mouse models of CRC and subcutaneous tumor mouse models were employed to assess the effects of LJ. Metagenomic, transcriptomic, and metabolomic analyses identified key metabolites and molecular targets, which were validated in AOM/DSS-induced antibiotic-treated (ABX) mouse models, MC38 xenografts, normal human intestinal epithelial cell lines, and CRC cell lines. LJ treatment significantly enhanced tumor necrosis factor (TNF) signaling pathways, and up-regulated apoptotic gene sets in tumor tissues. Thus, LJ shows considerable potential as a prophylactic and adjunctive therapeutic strategy for CRC management.

约氏乳杆菌（Lactobacillus johnsonii, LJ）在多种人类疾病中已展现出治疗潜力，但其对结直肠癌（colorectal cancer, CRC）的直接作用仍未得到充分探究。本研究通过多套综合性实验模型，评估了LJ对结直肠肿瘤的预防与治疗效果。研究采用氧化偶氮甲烷/葡聚糖硫酸钠（Azoxymethane/dextran sulfate sodium, AOM/DSS）诱导的CRC小鼠模型与皮下移植瘤小鼠模型，以考察LJ的干预效应。借助宏基因组学、转录组学与代谢组学分析，本研究鉴定出关键代谢物与分子靶点，并在AOM/DSS诱导的抗生素处理（ABX）小鼠模型、MC38细胞移植瘤模型、正常人肠上皮细胞系及结直肠癌细胞系中完成了验证。结果显示，LJ处理可显著增强肿瘤组织内的肿瘤坏死因子（tumor necrosis factor, TNF）信号通路，并上调凋亡基因集的表达。综上，LJ作为结直肠癌防治的预防策略与辅助治疗手段，具备可观的应用前景。

搜集汇总

背景与挑战

背景概述

该数据集基于一项研究，评估了Lactobacillus johnsonii（LJ）在结直肠癌预防和治疗中的效果，通过小鼠模型和细胞实验证明LJ能增强TNF信号通路并诱导肿瘤细胞凋亡。研究采用多组学分析（如宏基因组学、转录组学和代谢组学）来识别关键代谢物和分子靶点，为LJ作为结直肠癌管理策略提供了科学依据。