Nuclear Receptor Corepressor 1 (NCoR1) deletion changed the expression profile in cardiomyocytes

NCoR1 played an important role in regulating the expression of many genes. We were wondering what kinds of genes the NCoR1 would regulate in cardiomyocytes. Therefore we generated the cardiomyocyte-specific NCoR1 knockout mice (CMNKO). Then we tried to analyze gene expression of left ventricular from littermate control (LC) and CMNKO mice with an unbiased way. Therefore the samples from left ventricular were subjected RNA-sequencing. LV samples were collected from littermate control (LC) and cardiomyocyte-specific NCoR1 knockout (CMNKO) mice at the age of 2 months. Then the samples were sent for RNA sequencing.NCoR1在调控众多基因的表达过程中发挥关键作用。我们旨在探究NCoR1在心肌细胞（cardiomyocytes）中所调控的基因类别。为此，我们构建了心肌细胞特异性NCoR1敲除小鼠（cardiomyocyte-specific NCoR1 knockout mice，CMNKO）模型。随后，我们采用无偏倚分析手段，对同窝对照（littermate control，LC）小鼠与CMNKO小鼠的左心室组织进行基因表达分析。基于此实验设计，我们对左心室样本开展了RNA测序（RNA-sequencing）：采集2月龄的LC小鼠与CMNKO小鼠的左心室组织样本，随后将样本送检进行RNA测序。