Four-dimensional computational ultrasound imaging of brain hemodynamics

Four-dimensional ultrasound imaging of complex biological systems such as the brain is technically challenging because of the spatiotemporal sampling requirements. We present computational ultrasound imaging (cUSi), an imaging method that uses complex ultrasound fields that can be generated with simple hardware and a physical wave prediction model to alleviate the sampling constraints. cUSi allows for high-resolution four-dimensional imaging of brain haemodynamics in awake and anesthetized mice., In this study, we assessed cUSi via in-vivo imaging experiments in both anesthetized (n=1) and awake (n=1) adult C57BL6/J mice (8–10 weeks old). The national authority (Centrale Commissie Dierproeven, The Hague, The Netherlands; license: AVD1010020197846) granted ethical approval prior to the experiments, which were performed according to the institutional, national, and European Union guidelines. Data from both mice was collected using a custom-built 64-element matrix probe with an output modified by a plastic encoding mask. The probe was driven using a Hadamard encoded synthetic aperture transmission scheme transmitting continuously for 60 seconds at a rate of 32 kHz to acquire two data ensembles.  
This upload contains the reconstructed Power and Colour Doppler Volumes from the data ensembles of both the awake and anesthetized mice. These were formed by spatio-temporally filtering using a singular value decomposition the 60-second acquisitions to remove signal components originating ..., , # Four-Dimensional Computational Ultrasound Imaging of Brain Hemodynamics

The submission is comprised of 4 Nifti volumes: Awake_Color_Doppler, Awake_Power_Doppler, Anesthetized_Color_Doppler, and Anesthetized_Power_Doppler. The Nifti volumes can be visualised using a range of open source software (e.g., Slicer: ).

These four datasets respectively contain 3D information on the blood flow direction and power in the awake and anesthetized mouse brain and were used to generate the renderings and projections shown in Fig. 2 and Fig. S3 of the coupled manuscript.

The specific details for each data-set are as follows:

1\) Awake_Color_Doppler.nii - the color doppler volume formed from the awake mouse experiment containing the flow direction. The volume size is 176x156x180 with an isotropic voxel size of 40 microns the units indicate flow speed in m/s.

2\) Awake_Power_Doppler.nii - the power doppler volume formed from the awake mouse experiment containing the power in each voxel. The volu...