Transcriptome sequencing of thalidomide treated limb bud of chick embryo

Purpose: The aim of this study was to compare the differentially expressed genes under thalidomide treatment in limb buds. Methods: The trancriptome sequencing was performed using Illumina HiSeq 2500 ® platform. The sequence reads were aligned to the reference genome of chicken (Galgal4) downloaded from Ensembl Release 81 database. Alignment was performed using STAR (version 2.4.2a ). Expression estimation carried out using cufflinks program (version 2.2.1).Differential expression analysis carried using the cuffdiff program in cufflinks(version 2.2.1). Heat maps were generated for gene expression analysis using R statistical software. The mRNA profiles of thalidomide and vehicle control treated limb buds isolated from HH22 stage chick embryo were generated by deep sequencing, using Illumina HiSeq 2500 platform.