Spatial tissue proteomics analysis of laser capture microdisscted intestinal crypt compartment in celiac disease

Crypt hyperplasia is a key feature of celiac disease and various other small intestinal inflammatory conditions. By undertaking mass spectrometry-based tissue proteomics of the gut epithelial crypt zone, we found that active celiac disease is characterized by a strong interferon-γ (IFN-γ) signal. This signal, hallmarked by increased expression of MHC molecules, coincides with a markedly reduced expression of proteins associated with fatty acid metabolism. This dataset contains protoemics analysis of crypt comparment samples isolated by laser capture microdissection of tissue sections from FFPE duodenal gut biopsies from human subjects with and without celiac disease.