snRNA-seq of whole hearts from adult BL6 mice

We used snRNA-seq to investigate an entire adult mammalian heart of BL6 mice. Whole hearts were harvested from 4 male mice (12 weeks) after cervical dislocation. The hearts were pooled and nuclei isolated using the Nuclei PURE Prep isolation kit (Sigma-Aldrich, Darmstadt, Germany) according to the manufacturer's protocol. Sequencing was conducted by Genewiz (Leipzig, Germany) on the 10xGenomics system. Single nuclei were captured in droplet emulsions and snRNA-seq libraries were constructed as per the 10x Genomics protocol using GemCode Single-Cell 3' Gel Bead and Library V3 Kit. RNA was controlled for sufficient quality on an Agilent 2100 Bioanalyzer system and quantified using a Qubit Fluorometer.我们采用单细胞核RNA测序（snRNA-seq）技术，对BL6品系成年小鼠的完整心脏开展研究。实验取材于4只12周龄雄性BL6小鼠，经颈椎脱臼法处死后获取完整心脏。将获取的心脏组织混合后，按照生产商的操作流程，使用Nuclei PURE Prep分离试剂盒（Sigma-Aldrich，德国达姆施塔特）完成细胞核分离。测序工作由位于德国莱比锡的Genewiz公司依托10x Genomics平台开展：通过微滴乳液体系捕获单细胞核，并依照10x Genomics官方实验流程，使用GemCode单细胞3'凝胶磁珠与文库V3试剂盒构建snRNA-seq文库。随后使用Agilent 2100生物分析仪系统对RNA样品进行质量质控，确认其质量达标，并通过Qubit荧光计完成样品定量。