Additional file 1 of Long-term survival of asexual Zymoseptoria tritici spores in the environment

Additional file 1: Fig. S1 A As Fig. 1, percentage viable blastospores (spores with at least one live cell; assessed by live/dead staining with propidium iodide (PI)) over time in water; B, C examples of cells sampled and stained with PI after B 5 days in water or C 49 days in water. Yellow arrowheads indicate exemplar dead cells, flooded with PI stain. Green arrowheads indicate exemplar live cells, with staining at plasma membrane only. Red arrowheads indicate matter from dead, lysed cells. Fig. S2 As Fig. 1D, average number of cells per individual blastospore over time in water; B As Fig. 1C, example of cells sampled and stained with PI after 5 days in water; C exemplar blastospores stained with PI after 5 days in water; D exemplar blastospores stained with PI after 42 days in water. Note the reduction in cells per individual blastospore between B/C and D. Arrows in B indicate possible reasons for this change: yellow arrows indicate bud formation, which would initially produce one-cell blastospores (buds); white arrows indicate cells in the middle of a blastospore (i.e. non-terminal cells) which have died, potentially leading to splitting of the blastospore into two smaller individual blastospores. Fig. S3 Z. tritici cells survive at least 56 days after aqueous suspensions are allowed to dry. An example plate is shown bearing colonies of Z. tritici which arose after cells were suspended in water, and then suspensions allowed to dry completely on a sterile Petri dish surface before sealing the Petri dish for 56 days. Dried cells were then resuspended in water and an aliquot pipetted onto YPD agar and incubated at 18°C for 7 days. Colonies are clearly visible. Raw data underpinning each figure; raw gene expression data.