Inducible keratinocyte-specific Mettl3-deficient mice develop spontaneous skin lesions

We conducted RNA sequencing analysis on skin tissues obtained from spontaneous inflammatory skin lesions in keratinocyte-specific Mettl3 knockout (K14CreERT2;Mettl3fl/fl) mice and their controls (Mettl3fl/fl). In brief, we observed spontaneous skin inflammation in the tail of K14CreERT2;Mettl3fl/fl mice 2-3 weeks following tamoxifen injection. The objective of this study was to investigate the impact and underlying mechanism of Mettl3 in epidermal keratinocytes regarding skin inflammation Overall design: First, mice were injected with tamoxifen for five consecutive days to induce knockdown of mettl3 in epidermal keratinocytes. four weeks after the injection, skin samples were collected from the spontaneously inflamed areas of the tails of K14CreERT2;Mettl3fl/fl mice as well as from the normal tails of control mice (Mettl3fl/fl) and processed by liquid nitrogen milling, followed by RNA isolation.