Changes in Gene Expression Profiling and Phenotype in Aged Multidrug Resistance Protein 4-Deficient Mouse Retina

Multidrug resistance related protein 4 (MRP4) belongs to the C subfamily of ABC transporters and maintains cellular environment by functioning as an efflux pump. We aimed to obtain insights into the coeffects of MRP4 deficiency and ageing on the retina, and gene expression profiling using DNA microarray were performed. To find out genes significantly up- or downregulated in aged Mrp4-null mice, the comparison of microarray data using retinal samples was carried out between aged Mrp4-null mice and aged wild-type mice. Eight arrays are included. We isolated whole retina from aged (aged 46-55 weeks) Mrp4 knockout mice and wild-type mice. Then, RNA extracts were hybridized to Agilent SurePrint G3 Mouse Gene Expression Arrays 8x60K Ver. 2.0 in quadruplicate.