The effect of Israeli acute paralysis infection on honey bee brood care behavior

# IAPV effect on honey bee brood care behavior --- Honey bees were either experimentally infected with Israeli acute paralysis virus (IAPV) or treated with a control solution. Each bee was paint-marked for individual tracking of behaviors. The bees were grouped in sets of 10 to create three different types of social environment0% of bees exposed to IAPV, 100% of bees exposed, and a mixed-treatment group with 50% of bees exposed to IAPV. The bees were introduced to a larval queen, at which point they were observed for five minutes. The number of events and duration of behaviors such as "external-antennation" around the opening of the queen cell and entering inside the queen cell ("insertion") were recorded for each individual bee, as were the number of unique visitors ("responders") to the queen cell, and summed for the entire social group. The group-level responses were standardized by dividing by the number of living bees in the behavioral assay arena. Virus titers in the bees were measured with qPCR, and the dry head masses of nurse bees were also recorded. We found no evidence for a social immune response at the group level; however, individually, exposed bees interacted with the larva more frequently than their unexposed nestmates. While this could increase virus transmission from adults to larvae, it could also represent a hygienic response to increase grooming when an infection is detected. DishLevelData.csv refers to the group-level responses of the three different social environments (PercentExposed). There are columns for the duration of external-antennation (DurationA or simply DA), the number of external-antennation events (InteractionA or IA), the duration of insertion behaviors (DurationI or DI), the number of insertion behaviors (InteractionI or II), and the number of unique visitors to the queen cell (Responder# or R). These values were standardized by dividing by the number of living bees in the group at the time of recording (PopCorrectedDA, PopCorrectedDI, etc.). IndividualBeeLevelData.csv has data points for each bee used in the behavioral assays. Each row represents an individual bee, including the group the bee was observed with (Dish), the unique color marking (color), whether the bee was exposed to the virus or not (Treatment), and the social environment the bee was grouped in (PercentExposed). Similar to the dish level data, there are columns for the duration and number of antennation and insertion behaviors. If a bee did not respond at all (i.e. scored 0 on any of the behavioral metrics), it was notated as a NonResponder. If a bee scored higher than 0, it was designated as a Responder. NurseIAPVTiters.csv refers to the qPCR data for a subset of bees used in the behavioral assays, with references to the bees individual treatment as well as the social group it was a part of (Dish). Dataset includes the CT Mean and SD, the quantity mean and SD, and the log-transformed quantity mean values. Similarly, DryHeadMass.csv refers to the dry head mass (mg) data for a subset of bees. Script requires the R packages ggplot2, dplyr, lm4, Matrix, lattice, latticeExtra, performance, emmeans, and pwr. R script requires to the four named .csv files included with the dataset: DishLevelData.csv, IndividualBeeLevelData.csv, NurseIAPVTiters.csv, and DryHeadMass.csv.