RNA Sequencing Analysis of the gonadal adipose tissue and liver from PEP Wild Type, heterozygotes and knock out mice.. RNA Sequencing Analysis of the gonadal adipose tissue and liver from PEP Wild Type, heterozygotes and knock out mice.

Purpose: Peptidase D (PEP) plays a vital role in collagen turnover by degrading proline-containing dipeptides but its specific functional relevance in adipose tissue is unknown. The goal of this study is to compare transcriptome profiling (RNA-seq) in the gonadal adipose tissue and liver of wild type mice and mice where PEP has been ablated either partially (heterozygotes) or totaly (knock out). Overall design: We performed RNA-Sequencing of the liver of PEPWT, HET, and KO mice fed chow, control diet, Alios diet and HFD 45 %. Total liver RNA was extracted and quality checked (RIN >7). 1ug of RNA was used to construct barcoded sequencing libraries . All the libraries were validated and then multiplexed and sequenced on two lanes of Illumina HiSeq 4000.