Global analysis of binding sites of SF3B1 by CLIP-seq
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https://www.ncbi.nlm.nih.gov/sra/SRP417407
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资源简介：
SF3B1 is one of shared components of U2 and U12 snRNPs that are required for splicing of U2-type and U12-type introns, respectively. Interestingly, recurrent somatic mutations have been identified in the spliceosome components in human cancer. By using CLIP-seq for SF3B1, binding sites in human embryonic stem cells were determined. The most prominent binidng sites were at 3' splice sites on pre-mRNA. Interestingly, 5' and 3' ends of introns spliced out were enriched by CLIP. Overall design: CLIP-seq of endogenous SF3B1 by using anti-SF3B1-antibody (Bethyl, Catalog # A300-996A) and a rabbit IgG control.
创建时间：
2023-04-27
