BMP-2 induction in leptin-stimulated human primary chondrocytes increases collagen II expression.
收藏Figshare2016-02-23 更新2026-04-29 收录
下载链接:
https://figshare.com/articles/dataset/_BMP_2_induction_in_leptin_stimulated_human_primary_chondrocytes_increases_collagen_II_expression_/1618961
下载链接
链接失效反馈官方服务:
资源简介:
(A-B) Cells were kept as controls or stimulated with leptin for 10’, 30’, 1, 4, 8, and 24 h. (C-D) Cells were (C) pretreated with Noggin or (D) transfected with BMP-2-, Smad1-, or Smad5-specific siRNA and then kept as controls or treated with leptin for 8 and 24 h. (A) The mRNA expressions of col1a1, runx2, and osteocalcin were determined by real-time PCR. (B-D) The protein expressions of collagen II and COX2 were determined by Western blot. Data in (A) are mean ± SEM from three independent experiments. Results in (B-D) are representative of three independent experiments with similar results. *, P vs. untreated cells. #, P vs. siCL/leptin-treated cells.
创建时间:
2016-02-23



