Transcriptional profiling of striatum and cortex from a mouse model of Huntington's disease (Q140) crossed with Tcerg1 heterozygous mice [Tcerg1]

As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntingtons disease (HD) through various computational modeling efforts. Striatum and cortex were isolated from 6-month-old Tcerg1+/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 4 genotypes: WT, Tcerg1+/-, Q140, and Q140 X Tcerg1+/-, 8 replicates per genotype. RNASeq analysis was performed on samples from the striatum and cortex tissues of 6-month-old Tcerg1+/-, Q140 KI, Q140 X Tcerg1+/-, and wild-type mice. N = 8 per group (4F/4M).本数据集为加州大学洛杉矶分校（UCLA）与亨廷顿病研究基金会（CHDI）合作研究的一部分。研究团队通过多种计算建模手段筛选出123个与亨廷顿病（Huntington's disease, HD）相关的基因，并针对这些基因构建了基因敲除（knockout, KO）模型。 研究人员从6月龄的小鼠体内分离得到大脑纹状体与皮层组织，这些小鼠涵盖四种基因型：野生型（WT）、Tcerg1基因杂合敲除（Tcerg1+/-）、Q140敲入（knock-in, KI）亨廷顿病模型小鼠，以及Q140 KI与Tcerg1+/-的杂交子代。 针对上述组织样本，研究人员开展了转录组学分析（RNA测序，RNASeq），每个基因型设置8个生物学重复，每组样本量为8（4雌4雄）。