Subclinical mastitis lipidomics water buffalo

This study aimed to determine the lipidome of water buffalo milk with intramammary infection (IMI) by non-aureus staphylococci (NAS), also defined as coagulase-negative staphylococci (CNS), using an untargeted lipidomic approach. Non-aureus Staphylococci are the most frequently isolated pathogens from dairy water buffalo milk during mastitis. A total of 17 milk samples from quarters affected by NAS-IMI were collected, and the lipidome was determined by liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). The results were compared with the lipidome determined on samples collected from 16 healthy quarters. The study identified 1857 different lipids, which were classified into 14 classes. The abundance of 72 lipids changed in NAS-IMI milk as compared to healthy quarters. Significant changes occurred primarily in the class of free fatty acids. The results of this study provided first-time insight into the lipidome of dairy water buffalo milk. They demonstrated that NAS-IMI could induce changes in the lipidome of water buffalo milk.<b></b>