Tissue neutrophil heterogeneity

Purpose: The goals of this study are to compare bulk RNAseq profiles of tissue neutrophils. Methods: Bulk RNAseq of sorted neutrophils from bone marrow, spleen, blood, lung, peripheral blood, skin and intestine from wild-type (WT) mice, in triplicate, using Illumina. The sequence reads that passed quality filters were analyzed at the gene level with RSEM which mapped about 26 million sequence reads per sample to the mouse genome (build mm10/GRCm38) and identified 32328 genes. Examination of different transcriptomic profiles in neutrophils from 6 different tissues.研究目的：本研究旨在对比不同组织中性粒细胞的批量RNA测序（bulk RNAseq）表达谱。实验方法：采用Illumina测序平台，对野生型（WT）小鼠骨髓、脾脏、血液、肺、外周血、皮肤及肠道中分选得到的中性粒细胞进行三次生物学重复的批量RNA测序。对通过质量过滤的测序读段，使用RSEM在基因水平开展数据分析：每个样本约有2600万条测序读段比对至小鼠基因组（版本mm10/GRCm38），共鉴定出32328个基因。对来自6种不同组织的中性粒细胞的不同转录组谱进行分析。