WGS of Acinetobacter baumannii ATCC 17978 passaged in presence of antibiotics in mouse model

Acinetobacter baumannii ATCC17978 was infected oropharyngeally into Balb/c mice or in Balb/c mice depleted of neutrophils by cyclophosphamide treatment. Mice were treated with various antibiotics, and infection was allowed to proceed for 24 hours before lungs were removed and bacteria were pooled. The pooled bacteria were either sequenced en masse, or used to reinfect mice to enrich for bacteria resistant to the antibiotic. At least three parallel lines of bacteria were maintained during the sequential passaging in either the neutrophil replete or depleted mice. Data deposited are the total sequencing reads of the pool to identify mutants that arose after each passage, the relative abundance of each of the mutants. Passage number refers to the number of sequential passages. Cyclophosphamide refers to neutrophil depleted mice.