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Overexpression of miR-129-5p in thyroid carcinoma cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE19933
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TCP1 papillary thyroid carcinoma cells were plated at 100,000 cells/well in a 6-well plate and transfected with 10nM of a synthetic pre-miR-129-5p or a negative pre-miRNA using Lipofectamine RNAiMAX reagent. RNA samples were harvested at 24 and 48 hours post-transfection. Three independent experiments performed in dye-swap: miR-129-5p versus miR-Neg.
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2015-10-16
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