de novo heterochromatin domains are associated with reduced gene expression at both the ura4 and spbc2f12.03 loci.

1From Figure 1A and 1C.2Distance of the translation start site from the nearest edge of L5.3Number of independent RNA isolations included in analysis.4p-value resulting from comparison between ura4::ade6+ and ura4::L5-ade6+ strains.5Essential for viability [82].6Mutation in mlo3 results in a growth defect [83].7Essential for viability [84].Relative gene expression in the ura4 and spbc2f12.03 loci. Steady-state mRNA for genes in the ura4 (A�CH) and spbc2f12.03 (1�C7) loci were assayed via quantitative RT�CPCR and are reported relative to ura4::ade6+ control strains. The distance from the transcription start site to the nearest edge of L5 is indicated for each gene.