pcr Sequences

The repair of maxillofacial bone defects has long been a pivotal and challenging frontier in oral medicine research. This study aims to clarify the regulatory role and mechanism of miR-224-5p in the osteogenic differentiation of human dental pulp stem cells (hDPSCs), thereby laying a theoretical foundation for subsequent jaw defect repair.In this study, plasmid transfection was used to regulate the expression of miR-224-5p in hDPSCs, which were then treated with osteogenic induction medium to induce osteogenic differentiation. To determinethe mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN), qRT‒PCR was performed. A dual-luciferase assay was conducted to verify the targeting relationship between PTEN and miR-224-5p, and the regulatory role of the PI3K/AKT pathway was confirmed through rescue experiments.