Temporal Dynamics in Murine Cardiac Transcriptome following Myocardial Infarction

Myocardial infarction (MI) is a leading cause of heart failure with reduced ejection fraction. To overcome limitations of prior studies restricted to single timepoints, sexes, or partial tissue sampling, we generated a comprehensive, sex-inclusive, time-resolved transcriptional resource of the murine heart after non-reperfused MI. Male and female C57BL/6J mice were analyzed at baseline and 1, 4, 7, and 28 days post-MI, with ventricular dilation confirmed by echocardiography. Whole-heart profiling, including infarct and scar regions, enables detailed study of fibrotic and inflammatory processes. This dataset provides a rigorous community resource for investigating cardiac remodeling after MI. Overall design: Experimental design leveraged key/common timepoints and included both sexes with three biological replicates in each group. 15–20-week-old male and female C57BL/6J mice were subjected to no intervention (Day 0) or to non-reperfused myocardial infarction (MI) for 1, 4, 7, or 28 days. Echocardiography was performed before tissue harvest to confirm ventricular dilation. Harvested tissue was processed, snap frozen, and shipped for scRNA flex assay.