IFNa-induced gene expression in immortalized pig-tailed macaque CD4+ lymphocytes

In order to identify IFNa-induced host factor(s) that might inhibit replication of unadapted chimeric SIV/HIV-1 viruses (SHIVs), we measured IFNa-induced gene expression in immortalized pig-tailed macaque (Ptm) CD4+ lymphocytes. Triplicate cultures of Ptm lymphocytes were left untreated or treated with 1000 U/ml of IFNa. Twenty-four hours later, RNA was isolated, and RNA-seq libraries were prepared for sequencing. A total of 198 genes were found to be significantly differentially expressed (|logFC| = 0.585 & FDR 5%) upon IFNa treatment. 147/198 genes were found to be significantly upregulated and 51/198 genes were found to be significantly downregulated regulated upon IFNa treatment. Overall design: IFNa-induced gene expression in immortalized pig-tailed macaque CD4+ lymphocytes left untreated or treated with 1000 U/ml of IFNa.