Maternal salt diet has a marked impact on renal function in the pregnant dam.

Food and water intake were measured daily, values represent the average intake at day 20. A 24 h urine collection with paired blood sample enabled analysis of renal function. Osmolarity, creatinine and electrolytes were measured by an osmometer (Osmomat 030, Gonotec), auto-analyser (RX-IMOLA, Randox) and ICP-MS (XSeries II, Thermo Fisher, Ltd), respectively. Data are means ±SEM for n = 8 dams per dietary group and were analysed by 1-way ANOVA for an effect of treatment (Genstat v14). Statistical significance was accepted at P<0.05. NS, not significant. BW, body weight.