RNA-seq analysis of Trpm7-deficient muscle satellite cells

Muscle satellite cells (MuSCs), skeletal muscle-resident stem cells, are crucial for regeneration of myofibers. Molecular factors such as cations that trigger the transition of MuSCs from a quiescent to an active state remain largely unidentified. In this study, we identified TRPM7, a Mg2+-permeable ion channel, as a critical determinant for myofiber regeneration. We investigated gene profiles of Trpm7-deficient MuSCs to understand the role of TRPM7 during myogenesis. Our results suggest that TRPM7 governs the myogenesis, cell-cycle progression and mitochondrial biosynthesis in satellite cells to regulate crucial events (ie, activation, proliferation, and myogenesis) during skeletal muscle regeneration. control or Trpm7-/- MuSCs were isolated by FACS (fluorescence activated cell sorting) as a population of YFP+ve cells from Pax7-CreERT2; Rosa26-YFP mice. Total RNA samples were isolated from MuSCs 3 days post cardiotoxin (CTX) injury.