Summary of phenotypes of single and double mutants of V. cholerae O1 and their reverted or trans-complemented derivatives as analyzed by silver stained SDS-PAGE, phage sensitivity, and agglutination by anti-Ogawa typing serum.

aThe pattern of purified LPS from strains grown overnight in LB (unless otherwise indicated) was visualized by SDS-PAGE and silver staining and the phenotype of each strain was categorized as follows: (++) wild type appearance of O antigen substituted LPS, (+) altered levels or appearance of O antigen substituted LPS, (None) no O antigen substituted LPS was observed.bThe ability of ICP1 to infect these strains as evidenced by plaque formation with bacteria grown to OD600∼0.5 is indicated as follows: (++) clear plaques, (+) turbid plaques, (−) no plaques.cThe ability of purified LPS to inhibit plaque formation by ICP1 was determined using 10 µg of purified LPS and is indicated as the average log10 inhibition of two independent assays. n.d. not done.