hot springs metagenome Genome sequencing and assembly

The hot spring sediment represents a challenging environment, harboring a wide diversity of microorganisms producing a variety of enzymes with interesting properties. Here, we describe the metagenome composition of a microbial community in a hot spring sediment, as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases. Several candidate genes coding for xylan-degrading enzymes have been identified in the metagenomic dataset. Function-based screening of the metagenomic fosmid library revealed a novel GH11 family xylanase (XynA3) showing thermostable properties.