RNA seq of intrahepatic cholangiocarcinoma cell lines and normal bile duct epithelial cells

Our data uncovered the functional heterogeneity of CAFs during ICC metastasis and set the epigenetic regulator PCGF4 as the crucial effector Two patient-derived primary ICC cancerous cells (ICC-X1-PCGFH and ICC-X2-PCGF4L) exhibited distinct migration capabilities in the cell scratch assays. That is, ICC-X1-PCGFH cells migrated much faster than ICC-X2-PCGF4L. RNA-Seq analyses were carried out to identify the differentially expressed genes between these two lines and also compared to a normal intrahepatic biliary epithelial cell line (HIBEpic cells)