Isolation and identification of salivary glycoproteins recognized by the lectin DSA in patients with HBV-induced cirrhosis

Early diagnosis of liver cirrhosis induced by Hepatitis B virus (HBV) using salivary biomarkers has significant implications for early detection, screening, and prognosis, with the advantages of being non-invasive, easily accessible, and cost-effective. Identifying liver cirrhosis at an early stage can greatly improve patient outcomes by enabling timely intervention, reducing the risk of progression to hepatocellular carcinoma (HCC), and enhancing the effectiveness of therapeutic strategies. Our previous study has investigated the alterations in salivary glycopatterns related to HBVinfected chronic liver diseases, and found that the tri-/tetra-antennary N-glycancontaining glycoproteins recognized by the lectin Datura Stramonium Agglutinin (DSA) was closely related to HBV-induced cirrhosis. This project used 120 salivary samples which were divided into healthy control, hepatitis B, hepatic cirrhosis, and hepatocellular carcinoma to perform saliva microarray test (individual). Furtherly, the mixed saliva of each group was isolated by the lectin DSA and then identified by LC-MS, and the N-/O-glycans were released and identified by MALDI-TOF-MS.