Genome-wide mapping of Hif-1a binding sites in zebrafish [ChIP-seq]

Analysis of the binding sites of Hif-1a in both wild-type and von Hippel Lindau mutant zebrafish lines at 4dpf by ChIP linked next generation sequencing. The von Hippel Lindau mutant displays a systemic hypoxic response under normoxic conditions. Results show the extent of Hif-1a binding to the genome, and provide a basis for analysis of the transcriptional response to genetically induced hypoxia in zebrafish. Overall design: Analysis of the DNA binding sites of Hif-1a in both wild-type and von Hippel Lindau mutant zebrafish lines at 4dpf. The von Hippel Lindau mutant displays a systemic hypoxic response under normoxic conditions. Results show the extent of Hif-1a binding to the genome, and provide a basis for analysis of the dependency of the transcriptional response on Hif-1a in conditions of genetically induced hypoxia in zebrafish.