Gene expression profiles in rat lung following intratracheal instillation with impurity-free single-wall carbon nanotubes

To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish characterization of physicochemical properties of impurity-free single-wall carbon nanotubes (SWCNTs). We have prepared two types of dispersed the SWCNTs, namely relatively small bundles and a short linear shape (CNT-1) and large bundles and a long linear shape (CNT-2), and attempted to characterize time-dependent changes in the gene expression of lung tissues until 90 days after intratracheal instillation with SWCNTs suspensions at 0.4 mg injected dose per rat.为推进我们在转录水平评估人工合成纳米材料生物学效应的基因表达研究方法，本研究采用全基因组微阵列表达谱作为发现平台，以筛选可用于表征无杂质单壁碳纳米管（single-wall carbon nanotubes, SWCNTs）理化性质的潜在基因。我们制备了两种分散态SWCNTs：分别为相对较小的管束与短线性形态（CNT-1），以及较大的管束与长线性形态（CNT-2）；并向大鼠气管内滴注每只剂量为0.4mg的SWCNTs混悬液，尝试表征给药后90天内大鼠肺组织基因表达的时间依赖性变化。