Ptms unlocks chromatin, Fig 7

Brain glial progenitor cells in culture spontaneously transform into neoplastic cells with tumorigenic potential. Transcript analysis identified a histone binding protein up regulated during this process. An examination of its function when introduced into normal cells revealed a dramatic affects on chromatin, with decreased DNA methylation and induction of glioma identity genes.

These files contain individual fasta genomic sequences of rat brain glial cells (PC, files djt69), and human embryo kidney (HEK, files djt70, djt86) transfected with a plasmid expression vectors then treated with Ct conversion reagent (Zymo Research, Cat #D5001-1) to distinguish methylated and non-methyl CpG sites.

69-1 OPC x eGFP
69-2 OPC x Ptms
69-3 OPC x H1a
69-4 OPC x H1a,Ptms
70-2 HEK x Ptms
70-3 HEK x H1a
70-4 HEK x H1a Ptms
86-1 HEK x eGFP
86-2 HEK x Ptms
86-3 HEK x H1a
86-4 HEK x H1a, Ptms