Transcriptomic profile of recovered exogenous Tregs from injured bone, muscle, and skin of mice that were locally treated with Tregs, as well as heart, MLN and spleen from mice with MI that were systemically treated with Tregs.

Transcriptomic profile of recovered exogenous Tregs from injured bone, muscle, and skin of mice that were locally treated with Tregs, as well as heart, mediastinal lymph nodes (MLN) and spleen from mice with myocardial infarcts (MI) that were systemically treated with Tregs. Mice with tissue injuries were treated with exogenous Tregs that were sorted from spleens of Foxp3(IRES-mRFP) mice - C57BL6/J mice with bone, muscle and skin injuries were treated via hydrogel-mediated local delivery of Tregs soon after injury, while mice with myocardial infarcts (MI) were treated with systemic (intravenous) delivery of Tregs one day post-MI. Three days after Treg-delivery, the injured bone, muscle, and skin tissues, as well as heart, mediastinal lymph nodes and spleens from mice with MI were harvested, and the exogenous (delivered) Tregs were recovered by FACS sorting. These sorted exogenous Tregs recovered at day 3 post-Treg delivery (Day 3 recovered Tregs) were then used for mini-bulk RNA sequencing along with spleen Tregs before delivery as a control (Day 0 Spleen Tregs). Bone (cranial defect), muscle (volumetric muscle loss defect) and skin (full-thickness biopsy punch) injuries were induced in 10 week old male wild-type mice and treated with RFP+ Tregs sorted from Foxp3(IRES-mRFP) mice, locally delivered via a hydrogel directly polymerised at the site of injury. Control mice were treated with hydrogel alone. Heart injury (myocardial infarction, MI) was induced in 10 week old male wild-type mice by left coronary artery ligation. One day post-MI, the mice were administered with either RFP+Tregs, or PBS (control), via intravenous tail vein injection. In the heart injury model, the delivered Tregs homed to the heart, mediastinal lymph nodes and spleens of the mice with infarcts. These exogenous Tregs were recovered from all these 6 tissues of the mice, on day 3 post-Treg delivery, by cell sorting (n=3, each replicate represents one mouse). These sorted Day 3 exogenous Tregs, along with the Tregs before delivery, were used for mini-bulk RNA sequencing. ***Please note that the Series/Sample records have been updated on July 25, 2024****