Figure S1 - Small Ribosomal Protein RPS0 Stimulates Translation Initiation by Mediating 40S-Binding of eIF3 via Its Direct Contact with the eIF3a/TIF32 Subunit

Growth cessation caused by the RPS0A-depletion under non-permissive conditions is fully reversible by high copy expression of the wt RPS0A allele expressed form its endogenous promoter. Strain YID16 (rps0aΔ rps0bΔ YEpMET-RPS0A-U) bearing the RPPS0A WT allele under control of MET3 promoter was further transformed by YEpRPS0A-L (bearing the wt RPS0A allele under control of its own promoter) and the resulting transformant was spotted in four serial 10-fold dilutions on SD medium +/− methionine and incubated at 30°C for 2.5 days. (PDF)