Analysis of gene regulation by the TP63 isoforms deltaNp63alpha and deltaNp63beta using ChIPseq of p63 and H3K27ac

Our goal was to assess transcriptional changes of the colon carcinoma cell line HCT116 TP53-/- in response to expression of the TP63 isoforms deltaNp63alpha or deltaNp63beta. The goal was to understand how these C-terminal isoforms of p63 might differentially regulate gene expression. We also assessed how differential binding of these isoforms and how they affect local changes in H3K27ac. Overall design: ChIP-seq of 24hr induced (+dox) conditions for either a negative control, deltaNp63alpha, or deltaNp63beta HCT116 TP53-/- cell lines using an antibody targeting the deltaN-domain of p63. ChIP-seq of 24hr induced (+dox) conditions for either a negative control, deltaNp63alpha, or deltaNp63beta HCT116 TP53-/- cell lines using an antibody targeting H3K27ac (histone H3 lysine 27 acetylation)