TABLE 2 in Unravelling a Taxonomic Puzzle: Reinstating the South African Scolelepis capensis as distinct from Scolelepis squamata (Annelida: Spionidae)

TABLE 2. PCR Thermal cycling conditions for three genes: COI, 18S and 28S used to amplify Scolelepis specimens from South Africa, adapted from Surugiu et al. (2022). No. of cyclesGeneInitial denaturationDenaturationAnnealingExtensionFinal ExtensionCOI3894°C—3 mins94°C—30s46°C—45s72°C—1min72°C—5min18S4094°C—3 mins94°C—30s51°C—45s72°C—1.5min72°C—5min28S4094°C—5 mins94°C—20s52°C—45s72°C—1.5min72°C—5min