Effect of different immunosuppressors on cytokine-induced CXCL10 secretion in Hfsmc.

A, BXL-01-0029 (open triangles), elocalcitol (closed triangles), MTX (open squares), CsA (closed circles) and MeP (open circles) dose-dependently inhibited IFNγ+TNFα-induced CXCL10 secretion (closed squares, taken as 100%, ) after 24 h. Simultaneous fitting of the curves showed that BXL-01-0029 and elocalcitol retained the highest potency, as shown by the IC50s depicted in the inset of the figure. B, Neither infliximab (closed losangues, left) nor LEF (open losangues, right) exerted a dose-dependent inhibition of cytokine-induced CXCL10 secretion (closed squares, taken as 100%); IC50 s are reported in figure insets. C, At concentrations corresponding to the near therapy doses the inhibition by BXL-01-0029, not by elocalcitol, was significantly higher than MeP-, CsA- and MTX-induced ones (*P<0.05 or **P<0.01 vs. IFNγ+TNFα-induced secretion, #P<0.05 vs. all treatment except for elocacitol, °°P<0.01 vs. all other drugs). Results (mean±S.E.) are expressed as % of IFNγ+TNFα-induced CXCL10 secretion (taken as 100%) or % of inhibition. Data are obtained from five experiments with different cell preparations.