Gene expression profile at single cell level of adipocyte tissue macrophages from Apoe-null eWAT

Macrophages (ATM) are the predominant immune cells in WAT, but their heterogeneity and phenotypes are poorly defined during atherogenesis. The scavenger receptor CD36 mediates ATM crosstalk with other cells, driving chronic inflammation. Here, we conducted single-cell RNA sequencing on major WAT ATM subpopulations using a diet-induced atherosclerosis mouse model (Apoe-null). Overall design: Apoe-null or Apoe/Cd36 double-null mice on chow diet or high fat diet for 10 weeks were sacrificed, eWAT were isolated and CD45+ cells were selected for scRNA-seq analysis using 10X Genomics platforms