Expression of NSD3short-3xFLAG in Multiple Cell Lines
收藏NIAID Data Ecosystem2026-05-02 收录
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SGC Open Notebook Project to Characterize the HMTase NSD3 Exp018 Objective:To generate cell lines stably expressing NSD3-Short-3xFLAG in relevant cell backgrounds. These cell lines can be used to assay phenotypes associated with over-expression and to study NSD3-short protein-protein / protein-chromatin interactions. The lentiviral vectors used here have been modified by replacing an IRES bicistronic element with a T2A self-cleaving peptide sequence (cloning described in exp-016) for expression of NSD3short-3xFLAG and a puromycin selection marker.
创建时间：
2024-08-02
