Distribution of RNA polymerase II Ser5P, Histone 3 K27ac, and DNA in zebrafish embryos

This data set assesses the distribution of RNA polymerase II with serine 5 phosphorylation of the C-terminal domain repeat of subunit 1 (Pol II Ser5P), histone 3 lysine 27 acetylation (H3K27ac), and DNA in the nuclei of zebrafish embryos. Polymerase and histone 3 were labeled by immunofluorescence and DNA by JF646-Hoechst, microscopy images were acquired by 3-color STED microscopy and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.