Differential gene expression in CD137HI vs CD137LO human healthy donor monocytes

The cell surface glycoprotein CD137, which is also known as 4-1BB, belongs to the group of co-stimulatory immune receptors and is a member of the TNF receptor superfamily (TNFRSF). It is preferentially found on activated T-cells and regulatory T-cells but also innate immune cells such as natural killer cells, neutrophils, and monocytes can express CD137. In order to delineate properties of monocytes expressing CD137, RNAseq of CD137HI vs CD137LO expression monocytes was performed. Monocytes from three different healthy donors were purified via MACS and subsequently FACS-sorted into CD137HI and CD137LO populations followed by RNAseq analysis.