Human Immune Exhaustion Gene Expression Profile in C7R-CAR and CAR NK cells

The purpose of this experiment was two-fold. The first was to examine how the gene expression profile changes over time in C7R-CAR NK cells. C7R is a constitutively active IL-7 receptor that provides persistent activation of STAT5. The second was to examine the differential gene expression in C7R CAR NK cells or CAR NK treated with exogenous IL-15 (exIL15) after 2 weeks of stimulation. There are 9 experimental samples. Total RNA from C7R-GD2.CAR NK cells is represented at two different timepoints, the day 0 timepoint an day 14 timepoint. Each condition is represented by 3 matched donors. Total RNA was isolated at 14 from GD2.CAR NK cells treated with exogenous IL-15 to enable two week comparison of C7R and IL-15 signaling.