Psychedelic Zebrafish: the effects of LSD and MDMA on zebrafish behavior and physiology

In order to propel the acceptance of behavioral fish models, it is imperative to deconstruct the behavior of adult zebrafish into robust, quantifiable phenotypes. Our lab has extensively evaluated the novel tank test as a measure of affective behavior and the organization of locomotion. The use of zebrafish in pharmacological research has proved especially advantageous as it simplifies researchers’ abilities to hypothesize and observe behaviors comparable to those seen in rodents and humans.
Using the novel tank test, lysergic acid diethylamide (250 µg/L, 20 min exposure) and 3,4-Methylenedioxymethamphetamine (10, 40, 80, 120 mg/L, 20 min exposure) were given to # adult zebrafish. Traditional manual endpoints, automated video-tracking technologies and endocrine profiles were utilized and provided data that substantiates and quantifies behavioral, locomotive, and physiological domains.

为推动行为鱼类模型的普及接受度，将成年斑马鱼的行为分解为稳健且可量化的表型特征至关重要。本实验室已对新型水族箱测试进行了广泛评估，将其作为情感行为和运动组织结构的衡量标准。在药理学研究中，斑马鱼的使用特别具有优势，因为它简化了研究人员对与啮齿动物和人类行为可比的假设和观察的能力。通过新型水族箱测试，向#成年斑马鱼施用麦角酸二乙基酰胺（250 µg/L，20分钟暴露）和3,4-甲基苯丙二氧基甲基苯丙胺（10、40、80、120 mg/L，20分钟暴露）。采用传统手动终点、自动化视频跟踪技术和内分泌谱进行测量，所获得的数据证实并量化了行为、运动和生理领域的各项指标。