Seawater carbonate chemistry and circadian splicing patterns in the brain of fish

In this study, we examine the transcriptional patterns mediated by alternative splicing in driving the molecular response of fish to OA across their daily cycle. We assess the impact of environmental CO2 variation at four timepoints throughout a circadian (24-hr) day and aim to: (1) determine if the temporal patterns of splicing events in the brain in control conditions are altered by exposure to near-future predicted CO2 levels, and (2) gain a comprehensive understanding of splicing patterns under stable and fluctuating CO2 conditions by conducting pairwise comparisons between control and each CO2 condition separately for each time point. We reveal how splicing events may facilitate regulation of biological responses of fish to environmental CO2 variability across a circadian timeline.