Transcript profiling of the roots and leaves of opt3-2 mutant and wild type Arabidopsis

Illumina 100bp single end sequencing was used to profile the transcriptomes of wild type (Columbia) and opt3-2 mutant Arabdiopsis leaves and roots under hydroponic conditions ( 4 weeks old, bolting stage). Sequencing resulted in 381 million uniquely mapping reads accross 36 libraries. Each sample was barcoded and divided into three sequencing lanes, while each genotype/tissue combination is represented by 3 biological replicates consisting of 3-4 individual plants (2 genotypes x 2 tissues x 3 biological replicates x 3 technical replicates = 36 libraries). Our results show the leaves and roots of the opt3-2 mutant have different iron sensing transcriptional programs in place which is independent of the iron status of the plant. Overall design: Transcript profiling of the roots and leaves of opt3-2 mutant and wild type Arabidopsis