Table 1 in Endophytic Colletotrichum (Sordariomycetes, Glomerellaceae) species associated with Citrus grandis cv. " Tomentosa " in China

Table 1. Gene regions, respective primer pairs and PCR protocols used in the study.
GenePrimer pairOptimised PCR protocolsReferencesITSITS194 °C: 5 min (94 °C: 30 s, 53 °C: 30 s, 72 °C: 1 min) × 32 cycles, 72 °C: 10 minWhite et al. (1990)ITS4gapdhGDF94 °C: 5 min (94 °C: 30 s, 60 °C: 30 s, 72 °C: 1 min) × 32 cycles, 72 °C: 10 minGuerber et al. (2003)GDRchs-1CHS-79F94 °C: 5 min (94 °C: 30 s, 49 °C: 30 s, 72 °C: 1 min) × 32 cycles, 72 °C: 10 minCarbone and Kohn (1999)CHS-345Rhis3CYLH3F94 °C: 5 min (94 °C: 30 s, 53 °C: 30 s, 72 °C: 1 min) × 32 cycles, 72 °C: 10 minCrous et al. (2004)CYLH3RactACT-512F94 °C: 5 min (94 °C: 30 s, 54 °C: 30 s, 72 °C: 1 min) × 32 cycles, 72 °C: 10 minCarbone and Kohn (1999)ACT-783Rβ-tubulinBt2a94 °C: 5 min (94 °C: 30 s, 58 °C: 30 s, 72 °C: 1 min) × 32 cycles, 72 °C: 10 minGlass and Donaldson (1995)Bt2bgsGSF194 °C: 5 min (94 °C: 30 s, 60 °C: 60 s, 72 °C: 1 min) × 35 cycles, 72 °C: 30 minGuerber et al. (2003)GSR1