Effect of GDNF-Gel/HA-Mg nerve conduit on gene expression during peripheral nerve regeneration

In this study, glial cell line-derived neurotrophic factor (GDNF)- Gelatin methacryloyl (Gel)/hydroxylapatite (HA)-Mg nerve conduit was developed. the conduit was implanted in sciatic nerve defect model in SD rats to explore its role in repairing peripheral nerve defects. Regenerated nerve tissues from GDNF-Gel/HA-Mg group and negative control group were collected for RNA-seq, aiming to explore the potential molecular mechanism of GDNF-Gel/HA-Mg in peripheral nerve regeneration.本研究开发了胶质细胞源性神经营养因子（glial cell line-derived neurotrophic factor, GDNF）-甲基丙烯酸明胶（Gelatin methacryloyl, Gel）/羟基磷灰石（hydroxylapatite, HA）-Mg神经导管。将该导管植入SD大鼠坐骨神经缺损模型，以探究其修复周围神经缺损的作用。收集GDNF-Gel/HA-Mg组与阴性对照组的再生神经组织进行RNA测序（RNA-seq），旨在探索GDNF-Gel/HA-Mg促进周围神经再生的潜在分子机制。